pLXSN IkB alpha M
(Plasmid #12330)

• Depositing Lab: Inder Verma
• Publication: Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Backbone

• Vector backbone: pLXSN
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5900
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IkB alpha M
• Alt name: IkBaM
• Alt name: I kappa B alpha mutant
• Species: M. musculus (mouse)
• Mutation: Dominant-negative mutant. Many signal transduction pathways resulting in NF-B activation culminate in a serine phosphorylation of IkB on residues 32 and 36. Phosphorylation of the COOH-terminal PEST sequence has been implicated in constitutive turnover of IkB. We combined the NH2- and COOH-terminal phosphorylation mutants into a single cDNA for this plasmid.
• Entrez Gene: Nfkbia (a.k.a. Nfkbi)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCMX-IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment. pLXSN-IkB alpha M was constructed by blunt insertion of the Eco RV fragment from pCMX-IkBM into the Hpa I site of pLXSN.