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pLenti4 Blast RFP-PNRC1 W300A
(Plasmid #123298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti4
  • Modifications to backbone
    Zeocin resistance replaced with Blasticidin resistance
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PNRC1
  • Species
    H. sapiens (human)
  • Mutation
    W300A
  • Entrez Gene
    PNRC1 (a.k.a. B4-2, PNAS-145, PROL2, PRR2)
  • Tag / Fusion Protein
    • turboRFP (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Two TetO operators exist between CMV and RFP which may impact expression in the presence of TetR. The expression is unaffected in a standard transfection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti4 Blast RFP-PNRC1 W300A was a gift from Giovanni Tonon (Addgene plasmid # 123298 ; http://n2t.net/addgene:123298 ; RRID:Addgene_123298)

  • For your References section:

    Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G. EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. 10.15252/embj.201899179 PubMed 30373810
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