A102-GAP-BoCCS-GRM
(Plasmid #123286)

• Purpose: Expression of Glo3 ΔAmpH (1-459) in E.Coli
• Depositing Lab: Blanche Schwappach
• Publication: Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jcs.232124. doi: 10.1242/jcs.232124.

Backbone

• Vector backbone: PQE
• Total vector size (bp): 7215
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ADP-ribosylation factor GTPase-activating protein
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1346
• Entrez Gene: GLO3 (a.k.a. YER122C)
• Promoter: T5
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xma1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: tctggtatgccgtgcgtactgcggtgatca
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAACAAATCCAGAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry