gB gag LV
(Plasmid
#12328)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12328 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMDLg/pRRE
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegag/pol
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MutationThe CypA binding region of the WT gag was replaced by the CypA of the macrophage tropic HIV-1 variant Ba-L
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Good for infection of Simian cells. Please see author's map for additional information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gB gag LV was a gift from Inder Verma (Addgene plasmid # 12328 ; http://n2t.net/addgene:12328 ; RRID:Addgene_12328) -
For your References section:
Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells. Kootstra NA, Munk C, Tonnu N, Landau NR, Verma IM. Proc Natl Acad Sci U S A. 2003 Feb 4. 100(3):1298-303. 10.1073/pnas.0337541100 PubMed 12547912
Map uploaded by the depositor.
