pCEP4-BG505.DS-SOSIP.6mut-gp160
(Plasmid #123278)

• Purpose: Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10393
• Total vector size (bp): 12758
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 (BG505) Env
• Alt name: gp160
• Alt name: Env
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 2583
• Mutation: Codon-optimized synthetic gene; SOSIP mutations; Mutations L154M, Y177W, I201C, N300M, N302M, T320L, I420M, A433C
• GenBank ID: ABA61516.1
• Promoter: CMV
• Tag / Fusion Protein:
  • CD5 leader peptide (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry