pLenti PGK Puro GFP-LC3B G120A
(Plasmid #123242)

• Purpose: Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
• Depositing Lab: Robin Ketteler
• Publication: Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.

Backbone

• Vector backbone: pLenti PGK GFP Puro (w509-5)
• Backbone size w/o insert (bp): 7849
• Modifications to backbone: Removal of EGFP, insertion of EGFP-LC3B G120A between BamHI and SalI sites.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Microtubule-associated proteins 1A/1B light chain 3B
• Species: H. sapiens (human)
• Insert Size (bp): 378
• Mutation: Glycine 120 to Alanine
• GenBank ID: NM_022818.4
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Promoter: PGK
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP C F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pLenti PGK Puro GFP-LC3B G120A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry