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Addgene

pCEP4-Q842gp160-754*
(Plasmid #123231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10393
  • Total vector size (bp) 12394
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 (Q842.d12) Env
  • Alt name
    gp160
  • Alt name
    Env
  • Species
    Human immunodeficiency virus 1
  • Insert Size (bp)
    2223
  • Mutation
    Codon-optimized synthetic gene; Premature stop codon at position 754
  • GenBank ID
    AAM66242.1
  • Promoter CMV
  • Tag / Fusion Protein
    • CD5 leader peptide (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-Q842gp160-754* was a gift from Erik Procko (Addgene plasmid # 123231 ; http://n2t.net/addgene:123231 ; RRID:Addgene_123231)

  • For your References section:

    Conformational engineering of HIV-1 Env based on mutational tolerance in the CD4 and PG16 bound states. Heredia JD, Park J, Choi H, Gill KS, Procko E. J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19. 10.1128/JVI.00219-19 PubMed 30894475
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