pDEST-CMV-N-mCherry
(Plasmid #123215)

• Purpose: (Empty Backbone) N-terminal mCherry tag. Gateway destination vector for mammalian expression.
• Depositing Lab: Robin Ketteler
• Publication: Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.

Backbone

• Vector backbone: pcDNA-DEST53
• Backbone manufacturer: Life Technologies
• Backbone size (bp): 7720
• Modifications to backbone: Replacement of cycle3 GFP with mCherry between SacI restriction sites, loss of T7 promoter.
• Vector type: Mammalian Expression ; Gateway destination
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMVF_pCDNA3
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pDEST-CMV-N-mCherry.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry