pDONR223 LC3C no-stop
(Plasmid
#123208)
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PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
- Backbone size w/o insert (bp) 2796
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Vector typeGateway Entry Vector / Entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMicrotubule-associated proteins 1A/1B light chain 3C
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SpeciesH. sapiens (human)
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MutationStop codon removed
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GenBank IDNM_001004343.2
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Entrez GeneMAP1LC3C (a.k.a. ATG8J, LC3C)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 F
- 3′ sequencing primer M13 R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 LC3C no-stop was a gift from Robin Ketteler (Addgene plasmid # 123208 ; http://n2t.net/addgene:123208 ; RRID:Addgene_123208) -
For your References section:
Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. 10.1080/15548627.2019.1569925 PubMed 30661429