pSB165 - pL1R3_pMAS::rLUC-I::tMAS
(Plasmid #123198)

• Purpose: binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pICH47822
• Backbone manufacturer: Weber et al., 2011 (DOI:10.1371/journal.pone.0016765)
• Backbone size w/o insert (bp): 4372
• Vector type: Plant Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pMAS::rLUC-I::tMAS
• Species: Synthetic
• Insert Size (bp): 1682

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pSB165 - pL1R3_pMAS__rLUC-I__tMAS.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector for transient expression of a Renilla luciferase (rLUC; intron for avoiding expression in Agrobacteria). Carbenicillin might be used for selection of transformed Agrobacteria.