pSB161 - pL2_pSB90_2x35S::GUS::tMAS
(Plasmid #123197)

• Purpose: binary plant vector for transient expression of GUS (with introns)
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pSB90
• Backbone manufacturer: Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
• Backbone size w/o insert (bp): 5851
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2x35S::GUS::tMAS
• Species: Synthetic
• Insert Size (bp): 3134

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pSB161 - pL2_pSB90_2x35S__GUS__tMAS.gbk
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector for transient expression of GUS (contains two introns for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.