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pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS
(Plasmid #123196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB90
  • Backbone manufacturer
    Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
  • Backbone size w/o insert (bp) 5851
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2x35S::ORCA3::tMAS
  • Species
    Synthetic; Catharanthus roseus
  • Insert Size (bp)
    1721

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector for transient expression of ORCA3 from Chatharanthus roseus. ORCA3 is an activator of the C. roseus STR1 promoter. Can be used in combination with pSB137 for transactivation/repression assays.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123196 ; http://n2t.net/addgene:123196 ; RRID:Addgene_123196)

  • For your References section:

    EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings. Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, Lee-Parsons CWT. Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019. 10.3389/fpls.2019.00755 PubMed 31263474
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