pSB117 - pL2_pSB90_fLUC-I_V2
(Plasmid #123192)

• Purpose: binary plant vector for transient TYLCV V2 and fLUC (with intron) expression
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pSB90
• Backbone manufacturer: Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
• Backbone size w/o insert (bp): 5851
• Vector type: Plant Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
• Species: Synthetic
• Insert Size (bp): 4478

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pSB117 - pL2_pSB90_fLUC-I_V2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transient fLUC and TYLCV V2 (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agrob. through VirGN54D in the vector backbone