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pSB62 - pL0_STR (pro + 5U)
(Plasmid #123186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH41295
  • Backbone manufacturer
    Weber et al., 2011 (DOI:10.1371/journal.pone.0016765)
  • Backbone size w/o insert (bp) 2247
  • Vector type
    Plant Expression ; part for plant expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Catharanthus roseus STR1 promoter and 5'UTR
  • Alt name
    GenBank: Y10182.1
  • Species
    Catharanthus roseus
  • Insert Size (bp)
    590
  • Mutation
    No BpiI and BsaI sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus. Can be used as a control for transactivation experiments. Confirmed activation through ORCA3 and repression through ZCT1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB62 - pL0_STR (pro + 5U) was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123186 ; http://n2t.net/addgene:123186 ; RRID:Addgene_123186)

  • For your References section:

    EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings. Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, Lee-Parsons CWT. Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019. 10.3389/fpls.2019.00755 PubMed 31263474
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