pSB65 - pL0_tGFP-I (CDS1)
(Plasmid #123182)

• Purpose: Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pICH41308
• Backbone manufacturer: Weber et al., 2011 (DOI:10.1371/journal.pone.0016765)
• Backbone size w/o insert (bp): 2247
• Vector type: Plant Expression ; part for plant expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
• Species: Synthetic
• Insert Size (bp): 907
• Mutation: No BpiI and BsaI sites; codon-optimised for plants

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pSB65 - pL0_tGFP-I (CDS1).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Golden Gate (MoClo - Weber et al., 2011), CDS1 position, turboGFP (P. plumata) codon-optimised for plants with intron, U5 small nuclear ribonucleoprotein component (A. thaliana) for transient and stable expression in plants. (Moved to CDS1 position from pICSL50016; Engler et al., 2014)