Venus1-Y705F/S727A-STAT3
(Plasmid #123176)

• Purpose: Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
• Depositing Lab: Federico Herrera
• Publication: Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125. doi: 10.1096/fba.2019-00049. eCollection 2020 Feb.

Backbone

• Vector backbone: pcDNA 3.3 Topo
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5440
• Total vector size (bp): 8242
• Modifications to backbone: None
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT3 tagged with Venus1 (aa 1-157)
• Species: H. sapiens (human)
• Insert Size (bp): 2802
• Mutation: Double Y705F and S727A substitutions
• GenBank ID: BC014482.1
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Tag / Fusion Protein:
  • Venus1 (aa 1-158) for bimolecular fluorescence complementation assays (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry