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Addgene

Venus1-S727A-STAT3
(Plasmid #123174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.3 Topo
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5440
  • Total vector size (bp) 8242
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT3 tagged with Venus1 (aa 1-157)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2802
  • Mutation
    S727A substitution
  • GenBank ID
    BC014482.1
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Tag / Fusion Protein
    • Venus1 (aa 1-158) for bimolecular fluorescence complementation assays (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Venus1-S727A-STAT3 was a gift from Federico Herrera (Addgene plasmid # 123174 ; http://n2t.net/addgene:123174 ; RRID:Addgene_123174)

  • For your References section:

    Can asymmetric post-translational modifications regulate the behavior of STAT3 homodimers?. Letra-Vilela R, Cardoso B, Silva-Almeida C, Maia Rocha A, Murtinheira F, Branco-Santos J, Rodriguez C, Martin V, Santa-Marta M, Herrera F. FASEB Bioadv. 2020 Jan 27;2(2):116-125. doi: 10.1096/fba.2019-00049. eCollection 2020 Feb. 10.1096/fba.2019-00049 PubMed 32123861
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