Skip to main content
Addgene

pBT100-neo
(Plasmid #123137)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBT100
  • Backbone size w/o insert (bp) 1700
  • Total vector size (bp) 3000
  • Vector type
    Synthetic Biology ; Golden Gate donor vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neomycin, poly-A terminator
  • Promoter none

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GCCTTTGAGTGAGCTGATACCG
  • 3′ sequencing primer GAAAAGTGCCACCTAAATTGTAAGCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden gate donor vector which, in conjunction with pCALNL-EGFP-BsaI acceptor vector and two dsDNA recombinase targets, assembles to form a fluorescent EGFP transfectable reporter. Donor vector contains neomycin resistance cassette and poly-A terminator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT100-neo was a gift from David Liu (Addgene plasmid # 123137 ; http://n2t.net/addgene:123137 ; RRID:Addgene_123137)

  • For your References section:

    High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases. Bessen JL, Afeyan LK, Dancik V, Koblan LW, Thompson DB, Leichner C, Clemons PA, Liu DR. Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0. 10.1038/s41467-019-09987-0 PubMed 31028261
Related items:
Commonly requested with: