pET-Bxb1-His
(Plasmid #123132)

• Purpose: Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.
• Depositing Lab: David Liu
• Publication: Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0.

Backbone

• Vector backbone: pET
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 6600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bxb1 integrase
• Species: Mycobacterium smegmatis
• Insert Size (bp): 1500
• GenBank ID: 920117
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GAAACAAGCGCTCATGAGCCCGAA
• 3′ sequencing primer: GTCCCATTCGCCAATCCGGATATAG

Resource Information

• Supplemental Documents:
  • pET-BxB1-His.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry