pET-His-Cre
(Plasmid
#123128)
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PurposeBacterial expression plasmid for wild-type Cre with an N-terminal His-tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 123128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
- Backbone size w/o insert (bp) 5100
- Total vector size (bp) 6200
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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SpeciesP1 coliphage
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Insert Size (bp)1032
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GenBank ID2777477
- Promoter T7
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Tag
/ Fusion Protein
- 6x His tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GAAACAAGCGCTCATGAGCCCGAA
- 3′ sequencing primer GTCCCATTCGCCAATCCGGATATAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-His-Cre was a gift from David Liu (Addgene plasmid # 123128 ; http://n2t.net/addgene:123128 ; RRID:Addgene_123128) -
For your References section:
High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases. Bessen JL, Afeyan LK, Dancik V, Koblan LW, Thompson DB, Leichner C, Clemons PA, Liu DR. Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0. 10.1038/s41467-019-09987-0 PubMed 31028261
Map uploaded by the depositor.
