pDEST-CMV GFP-LC3B G120
(Plasmid #123116)

• Purpose: Expresses EGFP-LC3B G120 in mammalian cells.
• Depositing Lab: Robin Ketteler
• Publication: Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.

Backbone

• Vector backbone: pDEST-CMV-N-EGFP
• Backbone size w/o insert (bp): 6052
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Microtubule-associated proteins 1A/1B light chain 3B
• Species: H. sapiens (human)
• Insert Size (bp): 378
• Mutation: Deleted amino acids 121-125. Stop codon after G120.
• GenBank ID: NM_022818.4
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP C F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pDEST-CMV GFP-LC3B G120.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry