pDEST-CMV GFP-LC3B G120
(Plasmid
#123116)
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PurposeExpresses EGFP-LC3B G120 in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST-CMV-N-EGFP
- Backbone size w/o insert (bp) 6052
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMicrotubule-associated proteins 1A/1B light chain 3B
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SpeciesH. sapiens (human)
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Insert Size (bp)378
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MutationDeleted amino acids 121-125. Stop codon after G120.
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GenBank IDNM_022818.4
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Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP C F
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-CMV GFP-LC3B G120 was a gift from Robin Ketteler (Addgene plasmid # 123116 ; http://n2t.net/addgene:123116 ; RRID:Addgene_123116) -
For your References section:
Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. 10.1080/15548627.2019.1569925 PubMed 30661429