pDEST-CMV 3xFLAG-GABARAPL1-GFP
(Plasmid #123112)

• Purpose: Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1
• Depositing Lab: Robin Ketteler
• Publication: Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.

Backbone

• Vector backbone: pDEST-CMV-3xFLAG-gateway-EGFP
• Backbone size w/o insert (bp): 6111
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gamma-aminobutyric acid receptor-associated protein-like 1
• Species: H. sapiens (human)
• GenBank ID: NM_031412.2
• Entrez Gene: GABARAPL1 (a.k.a. APG8-LIKE, APG8L, ATG8, ATG8B, ATG8L, GEC1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xFLAG (N terminal on backbone)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMVF_pCDNA3
• 3′ sequencing primer: EGFP Nrev

Resource Information

• Supplemental Documents:
  • pDEST-CMV 3xFLAG-GABARAPL1-GFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry