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pDEST-CMV 3xFLAG-LC3A-GFP
(Plasmid #123106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123106 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-CMV-3xFLAG-gateway-EGFP
  • Backbone size w/o insert (bp) 6111
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Microtubule-associated proteins 1A/1B light chain 3A
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_032514.3
  • Entrez Gene
    MAP1LC3A (a.k.a. ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • EGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-CMV 3xFLAG-LC3A-GFP was a gift from Robin Ketteler (Addgene plasmid # 123106 ; http://n2t.net/addgene:123106 ; RRID:Addgene_123106)

  • For your References section:

    Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. 10.1080/15548627.2019.1569925 PubMed 30661429
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