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PurposeExpresses 3xFLAG-GABARAPL1 wild-type in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 123100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-tripleFLAG-Gateway
- Backbone size w/o insert (bp) 4711
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGamma-aminobutyric acid receptor-associated protein-like 1
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SpeciesH. sapiens (human)
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GenBank IDNM_031412.2
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Entrez GeneGABARAPL1 (a.k.a. APG8-LIKE, APG8L, ATG8, ATG8B, ATG8L, GEC1)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVF_pCDNA3
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV 3xFLAG-GABARAPL1 WT was a gift from Robin Ketteler (Addgene plasmid # 123100 ; http://n2t.net/addgene:123100 ; RRID:Addgene_123100) -
For your References section:
Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. 10.1080/15548627.2019.1569925 PubMed 30661429