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Addgene

zbFGF
(Plasmid #12309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fgf2
  • Alt name
    bFGF
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    465
  • Mutation
    Ala 11 mutated to Pro, Ser 55 mutated to Asn
  • Entrez Gene
    fgf2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    zbFGF was a gift from James Thomson (Addgene plasmid # 12309 ; http://n2t.net/addgene:12309 ; RRID:Addgene_12309)

  • For your References section:

    Feeder-independent culture of human embryonic stem cells. Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA. Nat Methods. 2006 Aug . 3(8):637-646. 10.1038/nmeth902 PubMed 16862139
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