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Addgene

phL1A-pcDNA3
(Plasmid #12307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    OneShot-Top10 (Invitrogen)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L1CAM
  • Alt name
    MIC5, SPG1, CAML1, CD171, HSAS1, N-CAML1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3994
  • Entrez Gene
    L1CAM (a.k.a. CAML1, CD171, HSAS, HSAS1, HYCX, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phL1A-pcDNA3 was a gift from Vance Lemmon (Addgene plasmid # 12307 ; http://n2t.net/addgene:12307 ; RRID:Addgene_12307)

  • For your References section:

    Molecular structure and functional testing of human L1CAM: an interspecies comparison. Hlavin ML, Lemmon V. Genomics. 1991 Oct . 11(2):416-23. 10.1016/0888-7543(91)90150-D PubMed 1769655
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