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Addgene

LV-indLS2
(Plasmid #123068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBOB
  • Backbone manufacturer
    Verma lab (Salk Institute)
  • Backbone size w/o insert (bp) 7800
  • Total vector size (bp) 14358
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase ; Fluorescence
  • Selectable markers
    luciferase or mStrawberry expression following Cre mediated recombination

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CreERT2 with intron
  • Species
    Synthetic; Bacteriophage P1
  • Insert Size (bp)
    2127
  • Mutation
    synthetic intron added as indicated
  • Promoter CAGGS (flanked by inverted lox66 and lox71 sequences)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ggcgttactatgggaaca
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    Luc2
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1659
  • Promoter CAGGS (after Cre mediated inversion)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer acattacctacgccgagt
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    mStrawberry
  • Species
    Discosoma sp.
  • Insert Size (bp)
    738
  • Promoter CAGGS (after Cre mediated inversion)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGATGAACTTCGAGGACG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-indLS2 was a gift from Kevin Brindle (Addgene plasmid # 123068 ; http://n2t.net/addgene:123068 ; RRID:Addgene_123068)

  • For your References section:

    Versatile and enhanced tumour modelling in mice via somatic cell transduction. Rodriguez E, Mannion L, D'Santos P, Griffiths M, Arends MJ, Brindle KM, Lyons SK. J Pathol. 2014 Mar;232(4):449-57. doi: 10.1002/path.4313. 10.1002/path.4313 PubMed 24307564
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