pBluescript SK - hTRF1
(Plasmid
#12306)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 12306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript SK -
-
Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2960
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehTRF1
-
Alt namehuman TTAGGG repeat binding factor 1
-
Alt name821
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1640
-
Entrez GeneTERF1P3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBluescript SK - hTRF1 was a gift from Titia de Lange (Addgene plasmid # 12306 ; http://n2t.net/addgene:12306 ; RRID:Addgene_12306) -
For your References section:
A human telomeric protein. Chong L, van Steensel B, Broccoli D, Erdjument-Bromage H, Hanish J, Tempst P, de Lange T. Science. 1995 Dec 8. 270(5242):1663-7. 10.1126/science.270.5242.1663 PubMed 7502076
Map uploaded by the depositor.