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Addgene

pWS932
(Plasmid #123055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Turbo Competent E. coli
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRD [3b]
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.

Please note: Plasmid contains an approximate 50/50 population of C and A nucleotides at basepair 662, which could potentially cause a S235Y mutation in PRD [3b]. Please screen colonies for the correct C nucleotide at this position.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWS932 was a gift from Tom Ellis (Addgene plasmid # 123055 ; http://n2t.net/addgene:123055 ; RRID:Addgene_123055)

  • For your References section:

    Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. 10.1016/j.cell.2019.02.023 PubMed 30955892
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