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Addgene

pBluescript SK +/- mTRF1
(Plasmid #12303)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK +/-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTRF1
  • Alt name
    mouse TTAGGG repeat binding factor 1
  • Alt name
    840
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • GenBank ID
    u65586
  • Entrez Gene
    Terf1 (a.k.a. Pin2, Trbf1, Trf1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript SK +/- mTRF1 was a gift from Titia de Lange (Addgene plasmid # 12303 ; http://n2t.net/addgene:12303 ; RRID:Addgene_12303)

  • For your References section:

    Comparison of the human and mouse genes encoding the telomeric protein, TRF1: chromosomal localization, expression and conserved protein domains. Broccoli D, Chong L, Oelmann S, Fernald AA, Marziliano N, van Steensel B, Kipling D, Le Beau MM, de Lange T. Hum Mol Genet. 1997 Jan . 6(1):69-76. 10.1093/hmg/6.1.69 PubMed 9002672
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