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Addgene

m2-26 mTRF2
(Plasmid #12300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2960
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTRF2
  • Alt name
    mouse TTAGGG repeat binding factor 2
  • Alt name
    1637
  • Insert Size (bp)
    2118
  • GenBank ID
    AF003000.1
  • Entrez Gene
    Terf2 (a.k.a. TRF2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    m2-26 mTRF2 was a gift from Titia de Lange (Addgene plasmid # 12300 ; http://n2t.net/addgene:12300 ; RRID:Addgene_12300)

  • For your References section:

    Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2. Broccoli D, Smogorzewska A, Chong L, de Lange T. Nat Genet. 1997 Oct . 17(2):231-5. 10.1038/ng1097-231 PubMed 9326950
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