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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPROEX-HTb
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7596
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp104
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneHSP104 (a.k.a. YLL026W)
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Tag
/ Fusion Protein
- his tag (tev cleavable) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Sac1 (not destroyed)
- 5′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDoug Hattendorf
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6920 pPROEXHsp104b was a gift from Susan Lindquist (Addgene plasmid # 1230 ; http://n2t.net/addgene:1230 ; RRID:Addgene_1230) -
For your References section:
Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter J, Lindquist S. Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. 10.1126/science.1098007 PubMed 15155912