pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID
(Plasmid #122982)

• Purpose: AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT
• Depositing Lab: Matthew Kennedy
• Publication: Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002.

Backbone

• Vector backbone: pAAV hSyn
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 6800
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCh-IRES-BoNT/B(1-146)-iLID
• Species: Synthetic
• Insert Size (bp): 2300
• Mutation: iLID contains long lived V416I mutation
• Promoter: human synapsin
• Tag / Fusion Protein:
  • mCh

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: actcagcgctgcctcagt
• 3′ sequencing primer: ccacatagcgtaaaaggagcaac

Resource Information

• A portion of this plasmid was derived from a plasmid made by: iLID was obtained from Dr. Brian Kuhlman's lab (University of North Carolina, Chapel Hill) BoNT/B was a gift from Dr Thomas Binz (Hannover Medical School)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry