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Addgene

pBAD-Flp
(Plasmid #122969)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122969 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCP20
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Flp recombinase
  • Insert Size (bp)
    1272
  • Promoter pBAD

Cloning Information

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    pCP20 was originally derived from CGSC https://cgsc2.biology.yale.edu/Strain.php?ID=140691

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBAD-Flp does not have detectable Flp recombinase activity when arabinose is not present in the growth medium

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-Flp was a gift from Lydia Freddolino (Addgene plasmid # 122969 ; http://n2t.net/addgene:122969 ; RRID:Addgene_122969)
  • For your References section:

    High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription. Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL. Cell Syst. 2019 Mar 27;8(3):212-225.e9. doi: 10.1016/j.cels.2019.02.004. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.004 PubMed 30904377