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Addgene

pcDNA3.1_PA-Cre-Y324F
(Plasmid #122961)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122961 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5354
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pcDNA3.1_PA-Cre-Y324F
  • Species
    Synthetic
  • Insert Size (bp)
    2049
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_PA-Cre-Y324F was a gift from Moritoshi Sato (Addgene plasmid # 122961 ; http://n2t.net/addgene:122961 ; RRID:Addgene_122961)

  • For your References section:

    A photoactivatable Cre-loxP recombination system for optogenetic genome engineering. Kawano F, Okazaki R, Yazawa M, Sato M. Nat Chem Biol. 2016 Dec;12(12):1059-1064. doi: 10.1038/nchembio.2205. Epub 2016 Oct 10. 10.1038/nchembio.2205 PubMed 27723747
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