pSV_238
(Plasmid #122941)

• Purpose: Expression of the repressor to control the insertional promoter.
• Depositing Lab: Attila Becskei
• Publication: Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi: 10.1126/sciadv.1700006. eCollection 2017 Jul.

Backbone

• Vector backbone: pRS303
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 4386
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rTetR.SUM1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 4700
• Promoter: Pret2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Resource Information

• Supplemental Documents:
  • pSV_238.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pRS303-SalI-Pret2.rTetR.SUM1.Tact1-NotI. Plasmid contains rTetR, a part of the tet system. Origin of the tet system: Gossen and Bujard, 1992.