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Addgene

pEC005
(Plasmid #122939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4805
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YML071C (Marked)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1992
  • Mutation
    YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC
  • Promoter [tetO2]4inPgal1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pRS306-KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YML071C_4silmut_RS_MfeI-EcoRI-Ter-NotI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEC005 was a gift from Attila Becskei (Addgene plasmid # 122939 ; http://n2t.net/addgene:122939 ; RRID:Addgene_122939)

  • For your References section:

    Multiplexed gene control reveals rapid mRNA turnover. Baudrimont A, Voegeli S, Viloria EC, Stritt F, Lenon M, Wada T, Jaquet V, Becskei A. Sci Adv. 2017 Jul 12;3(7):e1700006. doi: 10.1126/sciadv.1700006. eCollection 2017 Jul. 10.1126/sciadv.1700006 PubMed 28706991
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