pICE TRP1-URA3
(Plasmid
#122894)
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Purpose(Empty Backbone) TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneICE plasmid
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Vector typeYeast Expression ; Yeast Recombination Cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICE TRP1-URA3 was a gift from Randy Hampton (Addgene plasmid # 122894 ; http://n2t.net/addgene:122894 ; RRID:Addgene_122894) -
For your References section:
ICE plasmids: combining the ease of yeast recombination cloning with the stability of genomic integration. Flagg MP, Kao A, Hampton RY. Yeast. 2019 May 10. doi: 10.1002/yea.3400. 10.1002/yea.3400 PubMed 31074531