pJL009
(Plasmid
#122862)
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PurposeBinary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modules
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreenII
- Backbone size w/o insert (bp) 2740
- Total vector size (bp) 4807
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Vector typeGolden Gate compatible plant transformation vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name35S:MS2-SRDX:tRBCS
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SpeciesSynthetic
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Insert Size (bp)2067
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-SRDX coding sequence = 1542-2069bp
RBCS terminator = 2077-2722bp
BsaI site #1 = 2757-2762bp
BsaI site #2 = 2771-2776bp
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJL009 was a gift from Markus Schmid (Addgene plasmid # 122862 ; http://n2t.net/addgene:122862 ; RRID:Addgene_122862) -
For your References section:
CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants. Lee JE, Neumann M, Duro DI, Schmid M. PLoS One. 2019 Sep 26;14(9):e0222778. doi: 10.1371/journal.pone.0222778. eCollection 2019. 10.1371/journal.pone.0222778 PubMed 31557222