pMH204
(Plasmid #122860)

• Purpose: Binary expression vector containing 35S:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules
• Depositing Lab: Markus Schmid
• Publication: Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. doi: 10.1371/journal.pone.0222778. eCollection 2019.

Backbone

• Vector backbone: pGreenII
• Backbone size w/o insert (bp): 2740
• Total vector size (bp): 4933
• Vector type: Golden Gate compatible plant transformation vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 35S:MS2-VP64:tRBCS
• Species: Synthetic
• Insert Size (bp): 2193

Resource Information

• Supplemental Documents:
  • pMH204.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.

Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-VP64 coding sequence = 1542-2195bp
RBCS terminator = 2203-2848bp
BsaI site #1 = 2883-2888bp
BsaI site #2 = 2897-2902bp