pLVpuro-CMV-N-EGFP
(Plasmid #122848)

• Purpose: (Empty Backbone) N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.
• Depositing Lab: Robin Ketteler
• Publication: Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.

Backbone

• Vector backbone: pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR
• Backbone size (bp): 9000
• Modifications to backbone: Replacement of region between NheI and BstBI sites with EGFP and gateway cassette.
• Vector type: Mammalian Expression, Lentiviral ; Gateway destination
• Promoter: CMV
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Chloramphenicol selection prevents recombination of unmodified backbone. However, expression clones generated using this construct are prone to recombination, and must be grown using a recombination deficient strain.
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP C F
• 3′ sequencing primer: cPPT-rev

Resource Information

• Supplemental Documents:
  • pLVpuro-CMV-N-EGFP.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry