pAR17_StrepI_hFASh_H8_pET22b
(Plasmid #122846)

• Purpose: Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
• Depositing Lab: Martin Grininger
• Publication: Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 2018 Dec 20.

Backbone

• Vector backbone: pET22b
• Backbone manufacturer: Novagen/Merck Millipore
• Backbone size w/o insert (bp): 5340
• Total vector size (bp): 13008
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human FASN/ human fatty acid synthase
• Species: H. sapiens (human)
• Insert Size (bp): 7668
• Mutation: wildtype
• Entrez Gene: FASN (a.k.a. FAS, OA-519, SDR27X1)
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • Twin-Strep tag (N terminal on insert)
  • His8 tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 prom
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry