pMH186
(Plasmid
#122837)
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PurposeMS2-NLS (no stop codon) with GreenGate C-D flanking sequences
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMK-RQ
- Backbone size w/o insert (bp) 2149
- Total vector size (bp) 2800
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Vector typeGolden Gate compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMS2-NLS, codon-optimised for Arabidopsis
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SpeciesSynthetic
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Insert Size (bp)651
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Tag
/ Fusion Protein
- Nuclear localisation signal (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 380-385p
MS2-NLS coding sequence = 393-872bp
BsaI site #2 = 878-883bp
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH186 was a gift from Markus Schmid (Addgene plasmid # 122837 ; http://n2t.net/addgene:122837 ; RRID:Addgene_122837) -
For your References section:
CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants. Lee JE, Neumann M, Duro DI, Schmid M. PLoS One. 2019 Sep 26;14(9):e0222778. doi: 10.1371/journal.pone.0222778. eCollection 2019. 10.1371/journal.pone.0222778 PubMed 31557222