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Addgene

pMH220
(Plasmid #122836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2734
  • Total vector size (bp) 6874
  • Vector type
    Golden Gate compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
  • Species
    Synthetic
  • Insert Size (bp)
    4140
  • Mutation
    Contains D10A and H840A mutations

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is designed for Golden Gate cloning using the enzyme BsaI.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
dCas9 coding sequence = 2260-6399bp
BsaI site #2 = 6445-6450bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH220 was a gift from Markus Schmid (Addgene plasmid # 122836 ; http://n2t.net/addgene:122836 ; RRID:Addgene_122836)

  • For your References section:

    CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants. Lee JE, Neumann M, Duro DI, Schmid M. PLoS One. 2019 Sep 26;14(9):e0222778. doi: 10.1371/journal.pone.0222778. eCollection 2019. 10.1371/journal.pone.0222778 PubMed 31557222
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