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Addgene

pIRES2-EGFP-SHP2WT
(Plasmid #12283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SHP-2WT
  • Alt name
    PTP2c
  • Alt name
    PTP-1D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • GenBank ID
    NP_002825
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
  • Tag / Fusion Protein
    • Myc (not canonical: KLISEEDL) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Neel.BG Department of Medicine, Beth Israel Deaconess Medical Center, Boston,Massachusetts 02115, USA.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-EGFP-SHP2WT was a gift from Anton Bennett (Addgene plasmid # 12283 ; http://n2t.net/addgene:12283 ; RRID:Addgene_12283)

  • For your References section:

    The major vault protein is a novel substrate for the tyrosine phosphatase SHP-2 and scaffold protein in epidermal growth factor signaling. Kolli S, Zito CI, Mossink MH, Wiemer EA, Bennett AM. J Biol Chem. 2004 Jul 9. 279(28):29374-85. 10.1074/jbc.M313955200 PubMed 15133037
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