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Addgene

HPV16 E7 TCR
(Plasmid #122728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSGV1
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7400
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 cells may also be used.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HPV16 E7 TCR
  • Species
    Synthetic
  • Insert Size (bp)
    1800

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HPV16 E7 TCR was a gift from Christian Hinrichs (Addgene plasmid # 122728 ; http://n2t.net/addgene:122728 ; RRID:Addgene_122728)

  • For your References section:

    Engineered T cells targeting E7 mediate regression of human papillomavirus cancers in a murine model. Jin BY, Campbell TE, Draper LM, Stevanovic S, Weissbrich B, Yu Z, Restifo NP, Rosenberg SA, Trimble CL, Hinrichs CS. JCI Insight. 2018 Apr 19;3(8). pii: 99488. doi: 10.1172/jci.insight.99488. 10.1172/jci.insight.99488 PubMed 29669936
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