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PurposeExpress HPV16 E6 TCR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSGV1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7300
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsTop10 cells may also be used.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHPV16 E6 TCR
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SpeciesSynthetic
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Insert Size (bp)1800
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HPV16 E6 TCR was a gift from Christian Hinrichs (Addgene plasmid # 122727 ; http://n2t.net/addgene:122727 ; RRID:Addgene_122727) -
For your References section:
Targeting of HPV-16+ Epithelial Cancer Cells by TCR Gene Engineered T Cells Directed against E6. Draper LM, Kwong ML, Gros A, Stevanovic S, Tran E, Kerkar S, Raffeld M, Rosenberg SA, Hinrichs CS. Clin Cancer Res. 2015 Oct 1;21(19):4431-9. doi: 10.1158/1078-0432.CCR-14-3341. 10.1158/1078-0432.CCR-14-3341 PubMed 26429982