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Addgene

pSKAP_AE
(Plasmid #122707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKAP
  • Backbone manufacturer
    Shaw and Bourret
  • Backbone size w/o insert (bp) 4703
  • Total vector size (bp) 4728
  • Modifications to backbone
    Addition of AE barcode sequence. ATGAGGACGGAGACGTGATTGTAG
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Barcode AE
  • Insert Size (bp)
    25

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKAP_AE was a gift from Travis Bourret (Addgene plasmid # 122707 ; http://n2t.net/addgene:122707 ; RRID:Addgene_122707)

  • For your References section:

    Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella. Shaw JA, Bourret TJ. J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630. 10.3791/59630 PubMed 31132072
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