pSKAP
(Plasmid
#122702)
-
Purpose(Empty Backbone) Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneThis is a new backbone created by fusing the FRT-flanked kanamycin resistance gene of pKD13 into the MCS of pPCR Script Cam SK+
- Backbone size (bp) 4703
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSKAP was a gift from Travis Bourret (Addgene plasmid # 122702 ; http://n2t.net/addgene:122702 ; RRID:Addgene_122702) -
For your References section:
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella. Shaw JA, Bourret TJ. J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630. 10.3791/59630 PubMed 31132072
Map uploaded by the depositor.
Map uploaded by the depositor.
