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pBABE puro SDF-1 alpha
(Plasmid #12270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SDF-1 alpha
  • Alt name
    stromal cell-derived factor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Entrez Gene
    CXCL12 (a.k.a. IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    See Shirozu M et al., 1995, Genomics 28: 495-500.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 0.4kb BamHI/XhoI fragment of the human SDF-1 alpha cDNA was cloned into the BamHI/SalI site of the pBABE-puro vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro SDF-1 alpha was a gift from Bob Weinberg (Addgene plasmid # 12270 ; http://n2t.net/addgene:12270 ; RRID:Addgene_12270)

  • For your References section:

    Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA. Cell. 2005 May 6. 121(3):335-48. 10.1016/j.cell.2005.02.034 PubMed 15882617
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